**1. 基因工程驱动的适应性改造**
科研团队采用CRISPR技术对水稻基因组进行精确编辑,重点优化株型结构和抗逆基因。通过缩短节间距离使植株高度压缩至30厘米,配合气雾栽培系统实现每平方米种植密度提升400%。引入辐射抗性基因AtDREB2A和OsMYB55,增强作物对宇宙射线及月球表面昼夜温差的耐受性,实验显示"/>

通过跨学科的合作与交流,我们能够突破传统思维的边界,开拓新的研究领域。

——院长致词

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要实现月球殖民地的粮食自给,这种微型水稻品种的开发需结合多项前沿技术。根据现有研究,其可行性体现在以下几个方面:### 1. **月球农业环境适配技术**月球熔岩管道内部温度稳定在-20~30℃,相比

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Modern space exploration relies heavily on resupplies of food from Earth, but this tends to be largely pre-prepared meals that rarely contain fresh ingredients. To counteract the negative effects that the space environment can have on human health, it's important to have a reliable source of food rich in vitamins, antioxidants, and fibres.

The Moon-Rice project aims to develop the perfect crop for sustaining life in space for long-duration missions, such as the occupation of permanent bases on the Moon or on Mars. "Living in space is all about recycling resources and living sustainably," says Marta Del Bianco, a plant biologist at the Italian Space Agency. "We are trying to solve the same problems that we face here on Earth."

Dr Del Bianco explains that one of the major challenges is the current size of crops grown on Earth. Even many dwarf varieties of rice are still too big to be grown reliably in space. "What we need is a super-dwarf, but this comes with its own challenges," she says. "Dwarf varieties often come from the manipulation of a plant hormone called gibberellin, which can reduce the height of the plant, but this also creates problems for seed germination. They're not an ideal crop, because in space, you just don't have to be small, you must also be productive."

The Moon-Rice project is not just a solo effort by the Italian Space Agency and also involves the collaboration of three Italian Universities. "The University of Milan has a very strong background in rice genetics, the University of Rome 'Sapienza' specialises in the manipulation of crop physiology and the University of Naples 'Federico II' has an amazing heritage in space crop production," says Dr Del Bianco. "We started this four-year project nine months ago, so it's very much a work in progress, but the preliminary results we have now are really promising," says Dr Del Bianco.

"Researchers at the University of Milan are isolating mutant rice varieties that can grow to just 10 cm high, so they're really tiny and this is a great starting point," says Dr Del Bianco. "At the same time, Rome has identified genes that can alter the plant architecture to maximize production and growth efficiency." Additionally, since meat production will be too inefficient for resource and space-limited space habitats, Dr Del Bianco and her team are looking into enriching the protein content of the rice by increasing the ratio of protein-rich embryo to starch.

Dr Del Bianco's own personal focus is on how the rice plants will cope with micro-gravity. "We simulate micro-gravity on Earth by continually rotating the plant so that the plant is pulled equally in all directions by gravity. Each side of the plant gets activated continuously and it doesn't know where the up and down is," says Dr Del Bianco. "It's the best we can do on Earth because, unfortunately, doing experiments in real microgravity conditions, i.e. in space, is complex and expensive."

Not only can fresh food be more nutritious than pre-cooked and packaged space meals, but it has significant psychological benefits too. "Watching and guiding plants to grow is good for humans, and while pre-cooked or mushy food can be fine for a short period of time, it could become a concern for longer-duration missions," says Dr Del Bianco.

Recent studies reveal that microgravity triggers unique responses in plants: calcium信号通路和活性氧(ROS)水平会在短期微重力暴露中显著波动,而长期太空实验中植物会通过代谢重组和增强向性反应实现适应。这解释了为何项目组需要通过地面模拟设备(如回转器)反复验证水稻的力学响应。值得注意的是,国际空间站上的Veggie和APH系统已证明适当通风和水处理可缓解微重力对植物气体交换的间接影响,这对Moon-Rice的环境控制系统设计具有重要参考价值。

为提高蛋白质含量,团队借鉴了地球上的巨型胚胎突变体研究。数据显示,这类突变体的胚胎中粗蛋白含量可达普通水稻的1.3倍,且富含精氨酸、赖氨酸等必需氨基酸。通过CRISPR/Cas9基因组编辑技术定向改造OsPIN基因家族(该基因家族在重力感知中起关键作用),项目组试图同步优化植株形态和营养组成。这种技术路线与近期国际植物航天生物学界推崇的分子策略不谋而合。

Space exploration is a very demanding job, which requires astronauts to be in peak physical and psychological condition. "If we can make an environment that physically and mentally nourishes the astronauts, it will reduce stress and lower the chances of people making mistakes. In space, the best case of a mistake is wasted money, and the worst case is the loss of lives," says Dr Del Bianco.

The Moon-Rice project is not only beneficial for space explorers but will have useful applications for growing plants in controlled environments on Earth too. "If you can develop a robust crop for space, then it could be used at the Arctic and Antarctic poles, or in deserts, or places with only a small amount of indoor space available," says Dr Del Bianco.

This research is being presented at the Society for Experimental Biology Annual Conference in Antwerp, Belgium on the 9th July 2025.

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